miRNA-194 对人骨肉瘤细胞系SOSP-9607 转移的作用研究.

Objective: miRNA-194 were knocked down or overexpressed in Osteosarcoma Cell Line SOSP-9607 to investigate its effect on Metastasis of human Osteosarcoma which to provide the foundation of theory of miRNA-194 as a new target of osteosarcoma therapy. Methods: We transfected human osteosarcoma cell lines SOSP-9607 by using recombinant lentiviruses and divided the cells into three groups with different levels of miRNA-194. Then the stable transfected cells were used in transwell and wound healing assay. Results:1) The transfection and selection of miRNA-194 using recombinant lentiviral expression vector was finished correctly and different levels of miRNA-194 were got sucessfully. 2) Results in the transwell migration and invasion assay showed significantly lower numbers of OE SOSP-9607 cells (28.60± 4.36) and (21.25± 6.42) and significantly higher numbers of KD SOSP-9607 cells (132.60± 15.64; 115.76± 11.38) compared with other groups (p<0.05). The wound healing assay showed that cells in OE groups exhibited a significant decrease in migration rate compared to the other three groups(p<0.05). Conclusions: miRNA-194 could inhibit metastasis of Osteosarcoma Cell Line SOSP-9607. MiRNA-194 could be further explored as a potential target in Osteosarcoma diagnosis and therapy. [ABSTRACT FROM AUTHOR]