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Investigation of bacterial inactivation in apheresis platelets with 24 or 30 hours between inoculation and inactivation.
- Source :
-
Vox Sanguinis . Oct2016, Vol. 111 Issue 3, p226-234. 9p. 1 Diagram, 1 Chart, 5 Graphs. - Publication Year :
- 2016
-
Abstract
- Background and Objectives Blood Centre logistics, staffing and donor scheduling may be optimized if pathogen inactivation ( PI) of platelets can be delayed until Day 1, but bacteria may rapidly grow during this time. This study evaluates bacterial PI performed 24 and 30 h after collection. Materials and Methods PAS-3 platelet units were collected on the Amicus and subsequently inoculated (3-53 CFU/unit) with 1of 5 transfusion relevant bacterial species ( n = 3/organism). Units were then stored for either 24 ± 0·3 or 30 ± 0·3 h at 20-24°C with agitation, subsequently treated with amotosalen and UVA, and stored for 7 days. Samples were taken before and after inactivation, on Days 2, 5 and 7 for BacT/ ALERT testing, and on Days 5 and 7 for plate counts. Results All samples from units taken prior to inactivation either demonstrated positive plate culture counts, or, in untreated positive controls, were culture-positive during storage. All contaminated units treated with amotosalen and UVA 24 after inoculation were culture-negative on all days tested. With inactivation performed 30 h following inoculation, one of 15 units (1-of-3 replicates) was culture-positive with Klebsiella pneumonia (1 × 109 CFU/ml) by Day 5. Conclusion Photochemical treatment did not inactivate 1 of 15 units to sterility in apheresis platelets stored in PAS with a 30-h delay between contamination and treatment, but did inactivate 15 of 15 units with a 24-h delay. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00429007
- Volume :
- 111
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Vox Sanguinis
- Publication Type :
- Academic Journal
- Accession number :
- 118988456
- Full Text :
- https://doi.org/10.1111/vox.12410