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Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model.
- Source :
-
SpringerPlus . 11/3/2016, Vol. 5 Issue 1, p1-10. 10p. - Publication Year :
- 2016
-
Abstract
- Objectives: This study aimed to investigate the alteration of Neuropeptide Y (NPY) in the hypothalamus and its correlation with insulin, leptin and ghrelin during the development of a rat model of type 2 diabetes mellitus. Methods: The type 2 diabetes mellitus model was developed in diet-induced obesity (DIO) rats followed by the intraperitoneal injection of low-dose streptozotocin (STZ, 25 mg/kg). At four time points during the development of type 2 diabetes mellitus in rats, the fasting serum insulin, leptin, and plasma ghrelin were measured and the hypothalamic neuropeptide Y (NPY) content and mRNA expression were detected in the rats, which were divided into 4 groups: normal control (NC), DIO, DIO, and T2DM; the mRNA expression of OB-Rb, and GSH-R1a in the hypothalamus were also assayed. Results: During the development of the type 2 diabetes mellitus rat model, both the fasting serum levels of insulin and leptin (ng/ml) elevated significantly and the fasting plasma ghrelin concentration decreased the hypothalamic NPY (pg/mg) content significantly. NPY mRNA increased significantly in a time-dependent fashion while both the OB-Rb and the GHS-R1a mRNA of the hypothalamus decreased significantly. Hypothalamic NPY concentration was positively correlated with the changes in serum insulin and leptin and negatively correlated with plasma ghrelin. Conclusions: During the development of the type 2 diabetes mellitus rat model, the hypothalamic NPY content and NPY mRNA expression increased in a time-dependent manner, which was positively correlated with the changes of the serum insulin and leptin and negatively correlated with the plasma ghrelin. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21931801
- Volume :
- 5
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- SpringerPlus
- Publication Type :
- Academic Journal
- Accession number :
- 119232481
- Full Text :
- https://doi.org/10.1186/s40064-016-3555-9