Garlic, Cilantro and Chlorella's Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio).

Bioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the present study was carried out to compare histologically the aspect of intestine tissue of Prussian carp specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation.150 Prussian carps, with weight of 10-12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed). Cadmium toxicity and the potential protective effect of the three lyophilized products against the impact of cadmium toxicity were histopathologically assessed. For this purpose, fragments of intestine were removed and routinely processed at the end of experimental period and analyzed in light microscopy. A specific QuickPHOTO Micro 2.2 software has been used for the histological study. Tissue alterations were assessed using the histopathological score ranging from - to +++ depending on the degree and extend of lesions: (-) none, (+) mild occurrence, (++) moderate occurrence, (+++) severe occurrence. Our research findings show that Cd induces a significant increase in histopathological changes like vascular network hypertrophies and reach infiltrating leukocyte cells. In the same time, chlorella powder added to the fish diet, expressed the most effectiveness on the intestinal recovery of the cadmium-intoxicated fish followed by while cilantro and garlic powder. [ABSTRACT FROM AUTHOR]