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Serological Patterns and Molecular Characterization of Occult Hepatitis B Virus Infection among Blood Donors.
- Source :
-
Hepatitis Monthly . Oct2016, Vol. 16 Issue 10, p1-8. 8p. 2 Diagrams, 2 Charts. - Publication Year :
- 2016
-
Abstract
- Background: Hepatitis B infections, characterized by the presence of a viral genome without detectable hepatitis B surface antigen (HBsAg; Occult hepatitis B infection [OBI]), have been reported recently. Objectives: We performed serological and molecular characterization of OBI among blood donors at Jiangsu province blood center during years 2013 and 2014. Methods: All donor samples were routinely screened by double enzyme-linked immunosorbent assay (ELISA) for hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), Treponema pallidum (TP), and alanine aminotransferase (ALT). Single-reactive, nonreactive, and ALT-elevated samples were pooled or resolved by nucleic acid testing (NAT). Seromarkers were examined in HBsAg-/DNA+ samples. After 1 to 12 months of follow up, seromarkers were screened again to verify OBI samples. Results:We studied 157119 samples from blood donors. A total of 154397 ELISA nonreactive donor samples were identified, andHBVDNAwas detected in 81 samples; no samples were positive for HIV or HCV RNA. Hepatitis B virus viral loads in most donors were less than 20 - 200 IU/mL. There was only one HBsAg-positive sample. Eighty HBsAg-/DNA+ samples were evaluated further. Of these samples, 85% (68/80) were reactive for anti-HBc and 36.2% (29/800) were reactive for anti-HBc and anti-HBs; 11.3% (9/80) did not have any detectable serological markers. Twenty-nine donors were followed up. One was HBsAg ELISA positive, and of six seronegative donors, all had anti-HBc and anti-HBs, but were negative for DNA. Samples were HBV genotypes B, C and D. Mutations in the S region of HBV DNA included S114T, G119R, P120S, T125M, C139Y, T140I, C147W, T148A, A159V/G, E164D, V168A, and R169C. Conclusions: Overall, we found that OBI was rare, but that the prevalence of OBI was slightly higher in Jiangsu than in other areas of China. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1735143X
- Volume :
- 16
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Hepatitis Monthly
- Publication Type :
- Academic Journal
- Accession number :
- 119251873
- Full Text :
- https://doi.org/10.5812/hepatmon.40492