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Engineering Yarrowia lipolytica for efficient γ-linolenic acid production.
- Source :
-
Biochemical Engineering Journal . Jan2017 Part A, Vol. 117, p172-180. 9p. - Publication Year :
- 2017
-
Abstract
- γ-Linolenic acid (GLA) has various well-documented beneficial physiological effects and high biological significance. Because the natural supply of GLA is insufficient, microbial GLA production is a promising method for pharmaceutical and nutraceutical purposes. To establish and develop a biotechnological process for GLA production by Yarrowia lipolytica , the codon-optimized △6-desaturase from Mortierella alpina was introduced into this yeast under the control of the strong hp4d promoter. A recombinant Y. lipolytica strain was constructed, which produced 4.6% GLA in total fatty acids. By using a temperature-shift strategy of cultivation, consisting in preliminary growth at 28 °C followed by 6-day culture at 20 °C, optimal levels of dry cell weight (DCW), lipid content and GLA concentration were obtained from the recombinant strain: 18.55 g/L, 1.16 g/L and 71.6 mg/L, respectively. These DCW, lipid and GLA values were respectively 25.7%, 19.6% and 60.9% higher than those obtained in the control cultivation experiment at the standard constant temperature of 28 °C. This work demonstrates the excellent capacity of Y. lipolytica for GLA production, by combining metabolic engineering with a temperature-shift strategy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1369703X
- Volume :
- 117
- Database :
- Academic Search Index
- Journal :
- Biochemical Engineering Journal
- Publication Type :
- Academic Journal
- Accession number :
- 120408388
- Full Text :
- https://doi.org/10.1016/j.bej.2016.10.014