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Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation.
- Source :
-
Bioresource Technology . Mar2017, Vol. 227, p50-55. 6p. - Publication Year :
- 2017
-
Abstract
- Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes , which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of 60 Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4 mL H 2 /(g DW h). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301 mL H 2 /g glucose with the mutant was higher by 81.8% than that of 166 mL/g glucose with the wild strain. The peak hydrogen production rate of 27.2 mL/(L·h) with the mutant was higher by 40.9% compared with that of 19.3 mL/(L·h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 227
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 120672789
- Full Text :
- https://doi.org/10.1016/j.biortech.2016.12.033