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Transcription factor NF-YA promotes a malignant phenotype by upregulating fatty acid synthase expression.
- Source :
-
Molecular Medicine Reports . 2016, Vol. 14 Issue 6, p5007-5014. 8p. - Publication Year :
- 2016
-
Abstract
- Recent studies have revealed that increased expression of the alpha subunit of nuclear transcription factor Y (NF-YA) is associated with the malignant phenotype of various tumors. However, whether elevated expression of NF-YA promotes a malignant phenotype in osteosarcoma (OS), and the molecular mechanisms underlying this predicted effect is currently unknown. In the present study, small hairpin RNA (shRNA)-mediated knockdown of endogenous NF-YA significantly inhibited the migration and invasion capabilities of OS cells in vitro, whereas ectopic expression of NF-YA increased the migration and invasion capabilities of these cells. In addition, the induction of upregulated NF-YA expression on the malignant phenotype of OS cells was attenuated by silencing fatty acid synthase (FASN) expression. Furthermore, the expression level of FASN was increased by upregulating NF-YA, while decreased FASN expression was observed following NF-YA silencing in OS cells. The results of the present study suggest that NF-YA may promote a malignant phenotype in OS cells, in part, by activating the FASN signaling pathway, which may represent a promising target for the management of OS. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENOTYPES
*PHENOTYPES
*FATTY acid oxidation
*SYNTHASES
*GENETIC pleiotropy
Subjects
Details
- Language :
- English
- ISSN :
- 17912997
- Volume :
- 14
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Molecular Medicine Reports
- Publication Type :
- Academic Journal
- Accession number :
- 120714302
- Full Text :
- https://doi.org/10.3892/mmr.2016.5897