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High-level expression of a novel chromoplast phosphate transporter ClPHT4;2 is required for flesh color development in watermelon.
- Source :
-
New Phytologist . Feb2017, Vol. 213 Issue 3, p1208-1221. 14p. 1 Color Photograph, 2 Diagrams, 1 Chart, 4 Graphs. - Publication Year :
- 2017
-
Abstract
- • Chromoplast development plays a crucial role in controlling carotenoid content in watermelon flesh. Modern cultivated watermelons with colorful flesh are believed to originate from pale-colored and no-sweet progenitors. But the molecular basis of flesh color formation and regulation is poorly understood. • More chromoplasts and released carotenoid globules were observed in the red-fleshed fruit of the 97103 cultivar than in the pale-colored fruits of the PI296341-FR line. Transcriptome profiles of these two materials identified Cla017962, predicted as ClPHT4;2, was dramatically up-regulated during flesh color formation. High ClPHT4;2 expression levels were closely correlated with increased flesh carotenoid contents among 198 representative watermelon accessions. Down-regulation of ClPHT4;2 expression in transgenic watermelons reduced the fruit carotenoid accumulation. • ClPHT4;2 as a function of chromoplast-localized phosophate transporter was tested by heterologous expression into a yeast phosphate-uptake-defective mutant, western blotting, subcellular localization, and immunogold electron microscopy analysis. Two transcription factors, ClbZIP1 and ClbZIP2, were identified, which responded to ABA and sugar signaling to regulate ClPHT4;2 transcription only in cultivated watermelon species. • Our findings suggest that elevated ClPHT4;2 gene expression is necessary for carotenoid accumulation, and may help to characterize the co-development of flesh color and sweetness during watermelon development and domestication. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WATERMELONS
*ELOQUENCE
*ELOCUTION
*EXPRESSIVE behavior
*DELSARTE system
Subjects
Details
- Language :
- English
- ISSN :
- 0028646X
- Volume :
- 213
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- New Phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 120805721
- Full Text :
- https://doi.org/10.1111/nph.14257