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Establishment of an alcoholic fatty liver disease model in mice.
- Source :
-
American Journal of Drug & Alcohol Abuse . Jan2017, Vol. 43 Issue 1, p61-68. 8p. - Publication Year :
- 2017
-
Abstract
- <bold>Background: </bold>Alcoholic fatty liver disease (AFLD) defines an important stage in the progression of alcoholic liver disease (ALD), which is a major cause of morbidity and mortality worldwide.<bold>Objective: </bold>To establish a mouse model of AFLD.<bold>Methods: </bold>Male C57BL/6 mice were divided into the following two groups: (i) a control group, which was allowed free access to food and water and (ii) an alcohol-treated group, which was administered a 15% (v/v) alcohol solution instead of water. After 8-9 months of treatment, serum biochemical indexes, histopathological changes, liver triglyceride content, iron storage, and ferritin light chain protein expression were measured using an automatic biochemical analyzer, hematoxylin-eosin (HE) staining, a commercially available kit, Prussian blue staining, and Western blot analysis, respectively.<bold>Results: </bold>Compared with the control group, the alcohol-treated group displayed increased levels of serum LDH, ALT, and AST, decreased levels of ALB, and no significant change in levels of TP. Additionally, increased levels of serum TG, T-CHO, and LDL and decreased levels of serum GLU and HDL were observed in the alcohol-treated mice. HE staining showed that lipid vacuolization occurred in the livers of alcohol-treated mice. The alcohol-treated mice also exhibited increased liver triglyceride content. Moreover, Prussian blue staining and Western blot analysis demonstrated that chronic alcohol administration caused iron overloading of the liver.<bold>Conclusions: </bold>Chronic administration of 15% (v/v) alcohol in the drinking water over 8-9 months caused AFLD in mice. Our results establish an AFLD model that represents a promising tool for the future study of the progression of ALD. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FATTY liver
*ALCOHOLIC liver diseases
*DISEASE progression
*ANIMAL models of alcoholism
*MORTALITY of people with alcoholism
*PROTEIN expression
*THERAPEUTICS
*DISEASE risk factors
*IRON metabolism
*ANIMAL experimentation
*ASPARTATE aminotransferase
*BIOLOGICAL models
*CHOLESTEROL
*ETHANOL
*HIGH density lipoproteins
*LACTATE dehydrogenase
*LIPASES
*LIVER
*LOW density lipoproteins
*MICE
*OXIDOREDUCTASES
*OXIDATIVE stress
*ALANINE aminotransferase
Subjects
Details
- Language :
- English
- ISSN :
- 00952990
- Volume :
- 43
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- American Journal of Drug & Alcohol Abuse
- Publication Type :
- Academic Journal
- Accession number :
- 120859775
- Full Text :
- https://doi.org/10.1080/00952990.2016.1217539