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Etest® versus broth microdilution for ceftaroline MIC determination with Staphylococcus aureus: results from PREMIUM, a European multicentre study.
- Source :
-
Journal of Antimicrobial Chemotherapy (JAC) . Feb2017, Vol. 72 Issue 2, p431-436. 6p. - Publication Year :
- 2017
-
Abstract
- <bold>Objectives: </bold>To compare the concordance of ceftaroline MIC values by reference broth microdilution (BMD) and Etest (bioMérieux, France) for MSSA and MRSA isolates obtained from PREMIUM (D372SL00001), a European multicentre study.<bold>Methods: </bold>Ceftaroline MICs were determined by reference BMD and by Etest for 1242 MSSA and MRSA isolates collected between February and May 2012 from adult patients with community-acquired pneumonia or complicated skin and soft tissue infections; tests were performed across six European laboratories. Selected isolates with ceftaroline resistance in broth (MIC >1 mg/L) were retested in three central laboratories to confirm their behaviour.<bold>Results: </bold>Overall concordance between BMD and Etest was good, with >97% essential agreement and >95% categorical agreement. Nevertheless, 12 of the 26 MRSA isolates found resistant by BMD scored as susceptible by Etest, with MICs ≤1 mg/L, thus counting as very major errors, whereas only 5 of 380 MRSA isolates found ceftaroline susceptible in BMD were miscategorized as resistant by Etest. Twenty-one of the 26 isolates with MICs of 2 mg/L by BMD were then retested twice by each of three central laboratories: BMD MICs of 2 mg/L were consistently found for 19 of the 21 isolates. Among 147 Etest results for these 21 isolates (original plus six repeats per isolate) 112 were >1 mg/L.<bold>Conclusions: </bold>BMD and Etest have good overall agreement for ceftaroline against Staphylococcus aureus; nevertheless, reliable Etest-based discrimination of the minority of ceftaroline-resistant (MIC 2 mg/L) MRSA is extremely challenging, requiring careful reading of strips, ideally with duplicate testing. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STAPHYLOCOCCUS aureus infections
*CEFTAROLINE
*SOFT tissue infections
*DRUG resistance in bacteria
*AZTREONAM
*DIAGNOSIS
*ANTIBIOTICS
*COMMUNICABLE diseases
*COMPARATIVE studies
*RESEARCH methodology
*MEDICAL cooperation
*MICROBIAL sensitivity tests
*PNEUMONIA
*RESEARCH
*SKIN diseases
*STAPHYLOCOCCUS aureus
*EVALUATION research
*COMMUNITY-acquired infections
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 72
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 120929462
- Full Text :
- https://doi.org/10.1093/jac/dkw442