A development and an improvement of selectable markers in Pleurotus ostreatus transformation.

Authors :: Matsunaga, Yukari
Ando, Megumi
Izumitsu, Kosuke
Suzuki, Kazumi
Honda, Yoichi
Irie, Toshikazu
Source :: Journal of Microbiological Methods. Mar2017, Vol. 134, p27-29. 3p.
Publication Year :: 2017
Abstract: Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3 ± 0.6 transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7 ± 11.5 transformants/μg plasmid DNA. [ABSTRACT FROM AUTHOR]

Subjects :: *PLEUROTUS ostreatus
*STREPTOTHRICIN
*BIALAPHOS
*BASIDIOMYCETES
*MUSHROOMS
*PLASMIDS

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