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A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish.
- Source :
-
Biosensors & Bioelectronics . Jun2017, Vol. 92, p602-609. 8p. - Publication Year :
- 2017
-
Abstract
- Hydrogen sulfide (H 2 S) plays an important role as a signaling compound (gasotransmitter) in living systems. However, the development of an efficient imaging chemosensor of H 2 S in live animals is a challenging field for chemists. Herein, a novel peptide-based fluorescence chemosensor L -Cu was designed and synthesized on the basis of the copper chelating with the peptide ligand (FITC–Ahx–Ser–Pro–Gly–His–NH 2 , L ), and its H 2 S sensing ability has been evaluated both in living cells and zebrafish. The peptide backbone and Cu 2+ -removal sensing mechanism are used to deliver rapid response time, high sensitivity, and good biocompatibility. After a fast fluorescence quench by Cu 2+ coordinated with L , the fluorescence of L is recovered by adding S 2− to form insoluble copper sulfide in aqueous solution with a detection limit for hydrogen sulfide measured to be 31 nM. Furthermore, the fluorescence chemosensor L -Cu showed excellent cell permeation and low biotoxicity to realize the intracellular biosensing, L -Cu has also been applied to image hydrogen sulfide in live zebrafish larvae. We expect that this peptide-based fluorescence chemosensor L -Cu can be used to study H 2 S-related chemical biology in physiological and pathological events. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT proteins
*HYDROGEN sulfide
*ZEBRA danio
*CHEMORECEPTORS
*CHEMISTS
Subjects
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 92
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 121539562
- Full Text :
- https://doi.org/10.1016/j.bios.2016.10.050