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Improvement influenza HA2 DNA vaccine cellular and humoral immune responses with Mx bio adjuvant.

Authors :
Soleimani, Sina
Shahsavandi, Shahla
Maddadgar, Omid
Source :
Biologicals. Mar2017, Vol. 46, p6-10. 5p.
Publication Year :
2017

Abstract

Immunization with DNA vaccines as a novel alternative to conventional vaccination strategy requires adjuvant for improving vaccine efficacy. The conserved immunogenic HA2 subunit, which harbors neutralizing epitopes is a promising vaccine candidate against influenza viruses. In this study, for the first time we explore the idea of using host interferon inducible Mx protein to increase the immunogenicity of HA2 H9N2 influenza DNA vaccine. The potency and safety of the Mx adjuvanted-HA2 vaccine was evaluated in BALB/c mice by different prime-boost strategies. To assess the effect of the vaccination on the virus clearance rate, mice were challenged with homologous influenza virus. Administration of the adjuvanted vaccine and boosting with the same regimen could effectively enhance both humoral and cellular immune responses in treated mice. These data demonstrated that Mx as host defense peptide can be potentiated for improving influenza vaccine efficacy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10451056
Volume :
46
Database :
Academic Search Index
Journal :
Biologicals
Publication Type :
Academic Journal
Accession number :
121785232
Full Text :
https://doi.org/10.1016/j.biologicals.2016.11.004