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Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells.
- Source :
-
Archives of Oral Biology . Jun2017, Vol. 78, p135-143. 9p. - Publication Year :
- 2017
-
Abstract
- Objective The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). Design A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. Results Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. Conclusions The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00039969
- Volume :
- 78
- Database :
- Academic Search Index
- Journal :
- Archives of Oral Biology
- Publication Type :
- Academic Journal
- Accession number :
- 122371591
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2017.02.018