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Rapid and efficient separation of glycoprotein using pH double-responsive imprinted magnetic microsphere.
- Source :
-
Talanta . Jul2017, Vol. 169, p98-103. 6p. - Publication Year :
- 2017
-
Abstract
- As biomarkers of many diseases, glycoproteins are of great significance to clinical diagnostics. However, the determination of low abundant glycoproteins in complex biological samples without any pretreatment process is still a problem. In this study, a rapid and convenient separation method for highly efficient enrichment of glycoproteins is reported, based on pH double-responsive imprinted magnetic microspheres. Thin imprinted polymer shells were fabricated onto the surface of magnetic microspheres by free radical polymerization, using 2-(Dimethylamino) ethyl methacrylate as pH-sensitive monomer, 4-vinylphenylbronic acid as boronate affinity monomer, and ovalbumin (OVA) as template molecule. Combining the advantages of pH-sensitive monomer and boronate affinity monomer, rapidly capture-release of OVA could be modulated by changing solution pH. Moreover, high absorption ability (81.2 mg/g) was achieved within about 10 min. This study provided responsible way to imprint glycoproteins and showed great potential for glycoprotein detection in clinical diagnostic. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 169
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 122454848
- Full Text :
- https://doi.org/10.1016/j.talanta.2017.03.065