DNA Methylation Changes in Pleurotus eryngii Subsp. tuoliensis (Bailinggu) in Response to Low Temperature Stress.

The methylation sensitive amplified polymorphism (MSAP) was used to induce DNA methylation transformation in mushroom mycelia. DNA obtained from the mycelial stages of P. eryngii subsp. tuoliensis was digested with isoschizomers Msp I or Hpa II (mixture of EcoR I), the ability to digest the sequence CpCpGpG as influenced by their methylation state. The data analysis demonstrated that full-methylated and unmethylation modifications were primary and the hemi-methylated ratio was significantly lower. These results indicated that the pattern of CG hypermethylation is abundant in P. eryngii subsp. tuoliensis. All fragments that were differentially amplified upon low temperature induction illustrated the feasibility of MSAP in edible mushrooms. Moreover, this study confirmed that genetic and epigenetic changes in P. eryngii subsp. tuoliensis were induced under low temperature. [ABSTRACT FROM AUTHOR]