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Advances in organic polymer-based monolithic column technology for high-resolution liquid chromatography-mass spectrometry profiling of antibodies, intact proteins, oligonucleotides, and peptides.

Authors :
Eeltink, Sebastiaan
Wouters, Sam
Dores-Sousa, José Luís
Svec, Frantisek
Source :
Journal of Chromatography A. May2017, Vol. 1498, p8-21. 14p.
Publication Year :
2017

Abstract

This review focuses on the preparation of organic polymer-based monolithic stationary phases and their application in the separation of biomolecules, including antibodies, intact proteins and protein isoforms, oligonucleotides, and protein digests. Column and material properties, and the optimization of the macropore structure towards kinetic performance are also discussed. State-of-the-art liquid chromatography-mass spectrometry biomolecule separations are reviewed and practical aspects such as ion-pairing agent selection and carryover are presented. Finally, advances in comprehensive two-dimensional LC separations using monolithic columns, in particular ion-exchange × reversed-phase and reversed-phase × reversed-phase LC separations conducted at high and low pH, are shown. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219673
Volume :
1498
Database :
Academic Search Index
Journal :
Journal of Chromatography A
Publication Type :
Academic Journal
Accession number :
122580326
Full Text :
https://doi.org/10.1016/j.chroma.2017.01.002