Back to Search
Start Over
Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
- Source :
-
Journal of Immunology . 4/15/2017, Vol. 198 Issue 8, p3195-3204. 10p. - Publication Year :
- 2017
-
Abstract
- Helicobacter pylori, the dominant member of the human gastric microbiota, elicits immunoregulatory responses implicated in protective versus pathological outcomes. To evaluate the role of macrophages during infection, we employed a system with a shifted proinflammatory macrophage phenotype by deleting PPARĪ³ in myeloid cells and found a 5- to 10-fold decrease in gastric bacterial loads. Higher levels of colonization in wild-type mice were associated with increased presence of mononuclear phagocytes and in particular with the accumulation of CD11b+F4/80hiCD64+CX3CR1+ macrophages in the gastric lamina propria. Depletion of phagocytic cells by clodronate liposomes in wild-type mice resulted in a reduction of gastric H. pylori colonization compared with nontreated mice. PPARĪ³-deficient and macrophage-depleted mice presented decreased IL-10-mediated myeloid and T cell regulatory responses soon after infection. IL-10 neutralization during H. pylori infection led to increased IL-17-mediated responses and increased neutrophil accumulation at the gastric mucosa. In conclusion, we report the induction of IL-10-driven regulatory responses mediated by CD11b+F4/80hiCD64+CX3CR1+ mononuclear phagocytes that contribute to maintaining high levels of H. pylori loads in the stomach by modulating effector T cell responses at the gastric mucosa. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MACROPHAGES
*HELICOBACTER pylori
*COLONIZATION
*LIPOSOMES
*T cells
Subjects
Details
- Language :
- English
- ISSN :
- 00221767
- Volume :
- 198
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 122600782
- Full Text :
- https://doi.org/10.4049/jimmunol.1601902