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Cathepsin K modulates invasion, migration and adhesion of oral squamous cell carcinomas in vitro.
- Source :
-
Oral Diseases . May2017, Vol. 23 Issue 4, p518-525. 8p. 1 Diagram, 7 Graphs. - Publication Year :
- 2017
-
Abstract
- Objective Cathepsin K was initially discovered as an osteoclast-specific cysteine proteinase, but the enzyme is also expressed in various cancers including oral squamous cell carcinomas. This study aimed to clarify the function of cathepsin K in oral squamous cell carcinomas. Materials and Methods Expression levels of cathepsin K were examined in six types of cell carcinomas. Carcinomas overexpressing cathepsin K were constructed. Effects of cathepsin K overexpression and treatment with odanacatib, a specific cathepsin K inhibitor, on cell invasion, migration and adhesion were analysed. Results Different levels of cathepsin K were expressed in carcinomas. Cathepsin K was predominantly localised in lysosomes. Cathepsin K overexpression impaired the proliferation of carcinomas. Invasion analysis showed that cathepsin K overexpression enhanced invasion and migration of carcinomas, whereas inhibition of cathepsin K by odanacatib caused the opposite effects in carcinomas. Cathepsin K overexpression also increased cell adhesion and slightly increased surface expression of the adhesion receptor CD29/integrin β1. Conclusions The enhanced invasion of carcinomas resulting from cathepsin K overexpression is probably due to the increased cell migration and adhesion. Thus, cathepsin K is implicated not only in protein degradation but also in invasion, migration and adhesion of oral squamous cell carcinomas. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL lines
*LYSOSOMES
*MOUTH tumors
*SQUAMOUS cell carcinoma
*CYSTEINE
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 23
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 122636962
- Full Text :
- https://doi.org/10.1111/odi.12643