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Rapid detection of aldicarb in cucumber with an immunochromatographic test strip.
- Source :
-
Food & Agricultural Immunology . 2017, Vol. 28 Issue 3, p427-438. 12p. - Publication Year :
- 2017
-
Abstract
- A rapid, simple, and sensitive immunochromatographic strip test has been developed to detect aldicarb (ALD) in 0.01 M phosphate buffer solution (PBS) (pH 7.4) and cucumber samples. The test was developed with a sensitive monoclonal antibody specific for ALD, generated by immunizing BALB/c mice with a well-characterized ALD – keyhole limpet hemocyanin conjugate, produced in our laboratory. Under the optimized condition, the cut-off limits of test strip for ALD were found to be 25 ng/mL in 0.01 M PBS (pH 7.4) and 100 ng/mL in cucumber samples, and the highest standard relative deviation was 9.05%. Both results were obtained within 5 min. The data indicate that the method is sensitive, rapid, and specific, so this immunochromatographic test strip has utility for the rapid high-throughput screening of the pesticide, especially in food samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 28
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 122715070
- Full Text :
- https://doi.org/10.1080/09540105.2017.1293015