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Rapid and ultrasensitive detection of 3-amino-2-oxazolidinone in catfish muscle with indirect competitive enzyme-linked immunosorbent and immunochromatographic assays.
- Source :
-
Food & Agricultural Immunology . 2017, Vol. 28 Issue 3, p463-475. 13p. - Publication Year :
- 2017
-
Abstract
- In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic assay to detect the furazolidone metabolite, 3-amino-2-oxazolidinone (AOZ). The detection of AOZ was based on the AOZ derivative 3-([2-nitrophenyl]methyleneamino]-2-oxazolidinone (2-NPAOZ). 3-([(3-Carboxyphenyl)methylene]-amino)-2-oxazolidinone (2-CPAOZ) was used as the immunizing hapten and a conjugate of AOZ and glyoxylic acid (AOZ-A) was used as the coating hapten. The monoclonal anti-2-NPAOZ antibody, 5G12, was generated with a half-inhibitory concentration (IC50) of 0.2 ng/mL and a line of 0.06–0.66 ng/mL. Its cross reactivity with other analogues was less than 8%. Spiked catfish samples (0.1, 0.2, and 0.5 μg/kg) were analyzed with the proposed system. The ic-ELISA showed a recovery range of 86.2–118.5% and the intra-assay and inter-assay coefficients of variation ranged from 4.3% to 9.4%. Under the optimum assay conditions, the immunochromatographic assay had a visual cut-off value of 0.5 μg/kg in catfish samples. Both methods can be used to detect AOZ in catfish samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 28
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 122715073
- Full Text :
- https://doi.org/10.1080/09540105.2017.1297778