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Effect of CHFR and PARP-1 on the Proliferation and Apoptosis of B-cell non-Hodgkin's Lymphoma Raji Cells.
- Source :
-
Progress in Modern Biomedicine . Mar2017, Vol. 17 Issue 7, p1430-1436. 7p. - Publication Year :
- 2017
-
Abstract
- Objective: To investigate the effect of checkpoint with forkhead-associated (FHA) and RING finger domains (CHFR) with poly (ADP-ribose) polymerase 1 (PARP-1) on the proliferation and apoptosis of B-cell non-Hodgkin's lymphoma Raji cells. Methods: Quantitative real-time PCR (qRT-PCR) and western blot were used to detect the CHFR, PARP-1 mRNA and protein expressions in Raji cells treated by 5-Aza-dC and transfected by CHFR lentivirus. Cell proliferation was analyzed by a standard Cell Counting Kit-8 (CCK-8) assay, and the cell cycle changes were detected by flow cytometry. Results: The mRNA expression of CHFR in the 5-Aza-dC Raji group was significantly up-regulated compared with that in the control group (P < 0.01). PARP-1 levels were decreased in mRNA levels and the protein expression levels compared with control group (all P<0.05). The mRNA expression of CHFR in the ShRNA group was significantly down-regulated compared with that in the control and normal group (both P < 0.01). The mRNA and protein expression levels of PARP-1 were increased in ShRNA group compared with the control group and negative control group (P < 0.05). The CCK results suggested that the decrease in CHFR expression promoted the cell proliferation (P<0.05). And Flow cytometry results demonstrated that the cell apoptosis rate was decreased after CHFR silencing (P<0.05). Conclusion: These data suggested that CHFR might regulate the proliferation and apoptosis of B-cell non-Hodgkin's lymphoma Raji cells through PARP-1. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16736273
- Volume :
- 17
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Progress in Modern Biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 122782266
- Full Text :
- https://doi.org/10.13241/j.cnki.pmb.2017.08.007