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Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan.
- Source :
-
Emerging Infectious Diseases . Feb2004, Vol. 10 Issue 2, p304-310. 7p. 6 Charts, 2 Graphs. - Publication Year :
- 2004
-
Abstract
- Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests (neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR. With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus-based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10806040
- Volume :
- 10
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 12281365
- Full Text :
- https://doi.org/10.3201/eid1002.030731