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Antisense Oligonucleotides Inhibit Vascular Endothelial Growth Factor/Vascular Permeability Factor Expression in Normal Human Epidermal Keratinocytes.
- Source :
-
Journal of Investigative Dermatology . Apr97, Vol. 108 Issue 4, p523-526. 4p. - Publication Year :
- 1997
-
Abstract
- In psoriatic lesions, epidermal keratinocytes overexpress vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) and transforming growth factor alpha (TGF-α). TGF-α has been shown to induce VEGF/VPF in normal human epidermal keratinocytes <em>in vitro</em>. By using a 19-mer antisense phosphorothioate oligodeoxynucleotide (PS-ODN) complementary to bases 6-24 relative to the translational start site of the VEGF/VPF mRNA, the control sense and mismatched PS-ODNs, we examined modulation of VEGF/VPF induction by TGF-α <em>in vitro</em>. Normal human epidermal keratinocytes were treated with PS-ODNs and Lipofectin for 8 h prior to the addition of TGF-α. Inhibition was assayed at the level of secreted protein by capture ELISA and mRNA expression was assayed by Northern blot analysis. The anti-sense PS-ODN was capable of inhibiting VEGF/VPF RNA and protein to near-basal levels. This inhibition was concentration dependent. No effect was observed with the sense or mismatch con- trol PS-ODNs. These studies suggest that antisense oligonucleotide technology may be a potential therapy for the inhibition of angiogenesis associated with certain skin disorders such as psoriasis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KERATINOCYTES
*EPIDERMIS
*OLIGONUCLEOTIDES
*NEOVASCULARIZATION
*SKIN
*PSORIASIS
Subjects
Details
- Language :
- English
- ISSN :
- 0022202X
- Volume :
- 108
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Investigative Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 12289740
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12289740