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Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources.
- Source :
-
Journal of Antimicrobial Chemotherapy (JAC) . Jun2017, Vol. 72 Issue 6, p1589-1596. 8p. - Publication Year :
- 2017
-
Abstract
- <bold>Objectives: </bold>This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective bla SHV-12 -carrying plasmids and sequencing one of these plasmids completely.<bold>Methods: </bold>Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed bla SHV-12 -carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the bla SHV-12 genetic environment were analysed by PCR and sequencing. One IncI1 plasmid was sequenced completely using HiSeq 2500 and gap closure by PCRs and Sanger sequencing.<bold>Results: </bold>Among the 23 SHV-12-positive E. coli , some isolates from different sources showed the same characteristics: ST23/phylogroup A (human, dog, livestock), ST57/D (wild bird, chicken meat) and ST117/D (chicken meat, chicken). All bla SHV-12 genes were horizontally transferable via 30-120 kb plasmids of incompatibility groups IncI1 ( n = 17), IncK ( n = 3), IncF ( n = 1), IncX3 ( n = 1) and a non-typeable plasmid. IncK plasmids, indistinguishable in size and restriction patterns, were found in isolates from different sources (ST57/D, meat; ST131/B2, meat; ST57/B1, dog). The IncI1- bla SHV-12 -carrying plasmids were mostly assigned to plasmid ST (pST) 26 and pST3. Three plasmids showed novel pSTs (pST214, pST215). The majority of the IncI1 transformants exhibited resistance to β-lactams, chloramphenicol and streptomycin (in relation with a class 1 integron containing an estX - psp - aadA2 - cmlA1 - aadA1 - qacI gene cassette array), and to tetracycline. A novel bla SHV-12 environment was detected and whole plasmid sequencing revealed a Tn 21 -derived- bla SHV12 -ΔTn 1721 resistance complex.<bold>Conclusions: </bold>Results from this study suggest that the dissemination of bla SHV-12 genes occurs by vertical (clonal) and horizontal transfer, the latter mainly mediated through IncI1 multidrug-resistance plasmids. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ESCHERICHIA coli
*PLASMIDS
*REPLICONS
*POLYMERASE chain reaction
*NUCLEOTIDE sequencing
*MULTIDRUG resistance
*MEAT microbiology
*ANIMAL experimentation
*ANTIBIOTICS
*CHLORAMPHENICOL
*DNA
*DOGS
*FOOD microbiology
*GENES
*GENETICS
*GENOMES
*HYDROLASES
*MICROBIAL sensitivity tests
*POULTRY
*SEQUENCE analysis
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 72
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 123183614
- Full Text :
- https://doi.org/10.1093/jac/dkx024