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The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.
- Source :
-
PLoS ONE . 5/24/2017, Vol. 12 Issue 5, p1-14. 14p. - Publication Year :
- 2017
-
Abstract
- The human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhydrolase activities that depend on a critical threonine residue at position 19. Consistently, ASPG but not its T19A mutant showed cytotoxic activity in K562, NALM-6 and MOLT-4 leukemic cell lines but not in normal cells. Regarding the mechanism of action of ASPG, it was able to induce a significant apoptotic death in K562 cells. Taken together our data suggest that ASPG, combining different enzymatic activities, should be considered a promising anti-cancer agent for inhibiting the growth of leukemia cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ASPARAGINASE
*THREONINE
*CELL lines
*APOPTOSIS
LEUKEMIA genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 123207390
- Full Text :
- https://doi.org/10.1371/journal.pone.0178174