Simultaneous Determination of Isoline and Its Two Major Metabolites Using High-Performance Liquid Chromatography.

Discusses the development of a simple and reliable high-performance liquid chromatographic assay for a simultaneous determination of isoline, a potent hepatotoxic pyrrolizidine alkaloid. Analysis of the metabolism of isoline by rat and mouse microsomal enzyme systems; Role of hepatic esterase in the detoxification againstisoline-induced hepatotoxicity; Means of incubating rat liver microsomes with isoline as substrate.