生物化学综合性实验的设计与实现 -以亲和层析法纯化猪胰蛋白酶为例.

Biochemistry experiment is an important professional basic course in the field of life science. The experimental technology contained in the biochemistry experiment played a vital role in training students' basic skills, cultivating practical ability and innovation ability. Around the protein chemistry and enzymatic properties in the biochemistry, the experiments of chicken ovalin preparation, porcine trypsin preparation, porcine trypsin purification, and the determination of purified trypsin activity and chicken ovalin inhibitory activity were re-integrated. Therefore a comprehensive experiment including protein separation and purification, determination of protein content and enzyme activity was designed. The spectroscopy and chromatography were also used in the comprehensive experiment. Finally comprehensive designing content in the experiment was studied. The design can provide the reference for the research and development of comprehensive and designing experiments in the biochemistry experiment teaching. [ABSTRACT FROM AUTHOR]