Back to Search Start Over

Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules.

Authors :
Vasileva, Vanya
Zuojun Yue
Tanaka, Tomoyuki U.
Gierlinski, Marek
Etsushi Kitamura
O'Reilly, Nicola
Source :
Journal of Cell Biology. Jun2017, Vol. 216 Issue 6, p1609-1622. 14p.
Publication Year :
2017

Abstract

The initial kinetochore (KT) encounter with a spindle microtubule (MT; KT capture) is one of the rate-limiting steps in establishing proper KT-MT interaction during mitosis. KT capture is facilitated by multiple factors, such as MT extension in various directions, KT diffusion, and MT pivoting. In addition, KTs generate short MTs, which subsequently interact with a spindle MT. KT-derived MTs may facilitate KT capture, but their contribution is elusive. In this study, we find that Stu1 recruits Stu2 to budding yeast KTs, which promotes MT generation there. By removing Stu2 specifically from KTs, we show that KT-derived MTs shorten the half-life of noncaptured KTs from 48-49 s to 28-34 s. Using computational simulation, we found that multiple factors facilitate KT capture redundantly or synergistically. In particular, KT-derived MTs play important roles both by making a significant contribution on their own and by synergistically enhancing the effects of KT diffusion and MT pivoting. Our study reveals fundamental mechanisms facilitating the initial KT encounter with spindle MTs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219525
Volume :
216
Issue :
6
Database :
Academic Search Index
Journal :
Journal of Cell Biology
Publication Type :
Academic Journal
Accession number :
123483428
Full Text :
https://doi.org/10.1083/jcb.201608122