Tag identification of flue-cured tobacco varieties by multiplex PCR technique.

With 12 varieties of flue-cured tobacco as a template, the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized. Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR. The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR. Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately. [ABSTRACT FROM AUTHOR]