Back to Search
Start Over
MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1.
- Source :
-
Cellular Physiology & Biochemistry (Karger AG) . Jun2017, Vol. 42 Issue 1, p2171-2182. 12p. - Publication Year :
- 2017
-
Abstract
- Background/Aims: Atherosclerosis is a multifactorial chronic disease and is the main cause of death and impairment in the world. Endothelial injury and apoptosis play a crucial role in the onset and development of atherosclerosis. MicroRNAs (miRNAs) have been proven to be involved in the pathogenesis of atherosclerosis. However, studies of the functional role of apoptosis-related miRNAs in the endothelium during atherogenesis are limited. Methods: Cell injury and apoptosis were measured in five types of cells transfected with miR-1185 or cotransfected with miR-1185 and its inhibitor. Bioinformatics analysis and a luciferase reporter assay were used to confirm the targets of miR-1185. The effects of the targets of miR-1185 on endothelial apoptosis were determined using small-interfering RNA. Results: In this study, we first report that miR-1185 significantly promoted apoptosis in endothelial cells but not in vascular smooth muscle cells and macrophages. A mechanistic analysis showed that ultraviolet irradiation resistance-associated gene (UVRAG) and krev1 interaction trapped gene 1 (KRIT1), targets of miR-1185, mediated miR-1185-induced endothelial cell apoptosis. Conclusion: The results revealed the impact of miR-1185 on endothelial apoptosis, suggesting that miR-1185 may be a potential target for the prevention and treatment of atherosclerosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*APOPTOSIS
*EPITHELIAL cells
*ATHEROSCLEROSIS
*BIOINFORMATICS
*LUCIFERASES
Subjects
Details
- Language :
- English
- ISSN :
- 10158987
- Volume :
- 42
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cellular Physiology & Biochemistry (Karger AG)
- Publication Type :
- Academic Journal
- Accession number :
- 123745345
- Full Text :
- https://doi.org/10.1159/000475571