Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3X1X2X3Y1Y2 Sex Chromosomeystem

For brown howler monkeys ( Alouatta guariba clamitans ), diploid chromosome numbers varying from 2n = 45 to 2n = 52, with XX/XY, X1 X1 X2 X2 /X1 X2 Y, and X1 X1 X2 X2 X3 X3 / X1 X2 X3 Y1 Y2 sex chromosome systems have been described by mitotic studies but still await confirmation by meiotic analyses. We analyzed 3 male individuals sampled in the wild (in the municipality of Santa Maria, RS, Brazil) as well as 1 male and 1 female individual in cap tivity at the São Braz breeding center. Peripheral blood samples and testicular biopsies were taken. We found different diploid numbers for both sexes in somatic cells, 2n = 45,X1 X2 X3 Y1 Y2 in males and 2n = 46,X1 X1 X2 X2 X3 X3 in females, with 4 metacentric (9–12), 7 submetacentric (1–6, 8), and 9 acrocentric autosomal chromosome pairs (13–20, 22). X1 and X2 were submetacentric chromosomes, while X3, Y1, and Y2 were acrocentric ones. Spermatocyte microspreads were examined for synaptonemal complexes. Pachytene spermatocyte analysis was done to verify the chromosome number and morphologies observed in mitotic karyotypes. Immunodetection was performed using anti-SMC3 and anti-CREST antibodies. The presence of a sex chromosome pentavalent X1 X2 X3 Y1 Y2 in the males was confirmed by C-banding in metaphase I and by immunodetection in prophase I by the clear identification of 5 centromeres. The G-banded karyotype corresponded to that previously described for A. g. clamitans in the south of Brazil (Curitiba, Parana State, and Blumenau, Santa Catarina State) and for the Misiones Province, Argentina. [ABSTRACT FROM AUTHOR]