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A cost-effective process for the coproduction of erythritol and lipase with Yarrowia lipolytica M53 from waste cooking oil.
- Source :
-
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C . May2017, Vol. 103, p86-94. 9p. - Publication Year :
- 2017
-
Abstract
- Waste cooking oil (WCO), an oil-based substance that is produced from cooking, causes heavy burden to the environment and society. The coproduction of erythritol and lipase by Yarrowia lipolytica from this low-cost fatty matter was evaluated in this study. Compared with other oily substances, WCO was more favorable for erythritol and lipase coproduction, and the optimum concentration was 30.0 g/L. As an osmotic agent, 80.0 g/L of NaCl was required for the efficient biosynthesis of erythritol. The beneficial nitrogen source for the coproduction was ammonium oxalate, with a C/N ratio of 87:1. Moreover, key enzymes for erythritol synthesis were induced in WCO medium. The coproduction was then scaled up in a 5 L fermenter. The maximum concentration of erythritol (22.1 g/L) was obtained after 72 h, corresponding to a yield of 0.74 g/g, whereas the highest lipase activity reached 12.7 U/mL in 24 h. This study proposed a low-cost bioprocess for synthesizing high-value products using WCO. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FATS & oils
*LIPASES
*BIOSYNTHESIS
*SUGAR alcohols
*AMMONIUM
*COOKING
Subjects
Details
- Language :
- English
- ISSN :
- 09603085
- Volume :
- 103
- Database :
- Academic Search Index
- Journal :
- Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C
- Publication Type :
- Academic Journal
- Accession number :
- 123841681
- Full Text :
- https://doi.org/10.1016/j.fbp.2017.03.002