Back to Search
Start Over
Measuring fluorescence polarization with a dichrometer.
- Source :
-
Analytical Biochemistry . Sep2017, Vol. 532, p9-11. 3p. - Publication Year :
- 2017
-
Abstract
- A method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 532
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 123866963
- Full Text :
- https://doi.org/10.1016/j.ab.2017.04.001