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miR-215 promotes cell migration and invasion of gastric cancer by targeting Retinoblastoma tumor suppressor gene 1.
- Source :
-
Pathology - Research & Practice . Aug2017, Vol. 213 Issue 8, p889-894. 6p. - Publication Year :
- 2017
-
Abstract
- Background Gastric cancer (GC) is one of the most common malignant tumor and has high mortality worldwide. microRNAs (miRNAs) play critical roles in carcinogenesis. Previous studied showed that miR-215 was involved in tumorigenesis and progression. This study was designed to clarify the biological function of miR-215 in GC. Methods qRT-PCR was used to detect the miR-215 expression in GC tissues and 6 human GC cell lines (AGS, SGC-7901, NCI-N87, GES-1, MKN-45 and BGC-823) as well. Transwell assay was used to investigate the biological function of miR-215 in GC. Luciferase reporter assay was used to confirm its effect on the regulation of the target gene Retinoblastoma tumor suppressor gene 1 ( RB1 ). Results miR-215 was frequently up-regulated in GC tissues compared to adjacent non-tumor tissues and GC cell lines. miR-215 expression level was correlated with the progression of tumor invasion and tumor-node-metastasis (TNM) stage. Over-expression miR-215 in GC cell lines promoted cell migration and invasion. Besides, miR-215 could down-regulate the expression of RB1 in vitro via directly binding to its 3′-untranslated region (UTR), while the expression of RB1 would suppress the miR-215-indueced GC cell migration and invasion. Conclusions miR-215 promoted cell migration and invasion of gastric cancer by directly targeting RB1 . [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03440338
- Volume :
- 213
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Pathology - Research & Practice
- Publication Type :
- Academic Journal
- Accession number :
- 124289108
- Full Text :
- https://doi.org/10.1016/j.prp.2017.06.006