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Development of an immunochromatographic strip for detection of acetamiprid in cucumber and apple samples.
- Source :
-
Food & Agricultural Immunology . Sep2017, Vol. 28 Issue 5, p767-778. 12p. - Publication Year :
- 2017
-
Abstract
- An immunochromatographic strip was developed for detection of acetamiprid (AC) and cross-reactivity with thiacloprid (TC) in cucumber and apple samples. This detection system was developed based on a sensitive and specific monoclonal antibody produced in our laboratory. Under optimized conditions, the cut-off limits of the test strip for AC were found to be 1 and 0.25 ng/mL for TC in phosphate-buffered saline. Meanwhile, with simple preparation for detection cucumber and apple samples, the cut-off limits for AC were found to be 5 and 2.5 ng/mL for TC in cucumber samples. The immunochromatographic assay also revealed AC cut-off values of 30 and 15 ng/mL in apple samples. Each test can be evaluated within 5 min. The data indicate that the method is sensitive, rapid, specific, and has the advantages of simplicity; therefore, this immunochromatographic assay is a useful screening method for detection of AC and TC residues in cucumber and apple samples. [ABSTRACT FROM PUBLISHER]
- Subjects :
- *THIACLOPRID
*MONOCLONAL antibodies
*CHROMATOGRAPHIC analysis
*CUCUMBERS
*APPLES
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 28
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 124290344
- Full Text :
- https://doi.org/10.1080/09540105.2017.1312294