XCT-790 对沉默DKK1、Sost 腺病毒载体转染MG63 细 胞中OPG、CTGF、FGF2、TNFα 的影响研究.

Objective To investigate the effect of EＲＲα inhibitor ( XCT-790 ) on silencing Dickkopf1 ( DKK1 ) ，sclerostin ( Sost) reconmbinant adenovirus vector，and the related proteins OPG，CTGF，FGF2，and TNFα in the transfection MG63 cells． Methods MG63 cells were divided into control group，silence DKK1 group，silence Sost group，silence ( DKK1 + Sost) group， XCT-790 dispose no-load adenovirus group，XCT-790 dispose silence DKK1 group，XCT-790 dispose silence Sost group，and XCT-790 dispose silence ( DKK1 + Sost) group． The silencing DKK1 or Sost reconmbinant adenovirus vector was transfected to MG63 cells，respectively． The expression levels of related proteins OPG，CTGF，FGF2，and TNFα were detected with Western blotting in MG63 cells． Ｒesults ( 1) Compared with control group，the expression levels of OPG，CTGF，FGF2 increased in silence DKK1，Sost，and DKK1 + Sost group ( P ＜ 0. 05 ) ，while the expression level of TNFα decreased ( P ＜ 0. 05 ) ． The expression levels of OPG，CTGF，and FGF2 decreased ( P ＜ 0. 05) ，while TNFα increased ( P ＜ 0. 05) ，when XCT-790 intervened MG63 cells． ( 2) Compared with silence DKK1，Sost，and DKK1 + Sost group，the increased expression levels of OPG，CTGF， FGF2 were impaired ( P ＜ 0. 05) and the decreased expression level of TNFα increased ( P ＜ 0. 05) in XCT-790 dispose silence DKK1，Sost，and DKK1 + Sost groups． Conclusion XCT-790 decreases the expression levels of OPG，CTGF，and FGF2， increases the expression level of TNFα． It also regulates the changed expression levels of OPG，CTGF，FGF2，and TNFα in silencing DKK1，Sost，and DKK1 + Sost MG63 cells． [ABSTRACT FROM AUTHOR]