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Purification and characterization of a high salt-tolerant alginate lyase from Cobetia sp. WG-007.
- Source :
-
Biotechnology & Applied Biochemistry . Jul/Aug2017, Vol. 64 Issue 4, p519-524. 6p. - Publication Year :
- 2017
-
Abstract
- An alginate lyase producing bacterial strain, Cobetia sp. WG-007, was isolated and identified from rotting seaweed. The alginate lyase, Aly-W02, was purified by procedures of ultrafiltration, Q-Sepharose Fast Flow, Phenyl Sepharose 6 Fast Flow, and Superdex-G100 with specific activity of 21,285.5 U/mg. Aly-W02 had an apparent molecular mass of 35 kDa. It exhibited maximum activity at 45 °C in 50 mM sodium phosphate buffer (pH 8.5). This alginate lyase was stable in the pH range of 6.0-8.5. Among the tested metal ions, the addition of K+, Na+, and Mg2+ ions can enhance the enzyme activities, while Ba2+, Ni+, Cu2+, Mn2+, Zn2+, Ag+, and ethylenediaminetetraacetic acid decreased the activities. It displayed high salt-tolerant ability; 0.8 M NaCl or 1.5 M KCl significantly enhanced the enzyme activity. Furthermore, Aly-W02 mainly released disaccharide, trisaccharide, and tetrasaccharid from alginate. It showed potential in producing low molecular weight alginate oligosaccharides. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08854513
- Volume :
- 64
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biotechnology & Applied Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 124751842
- Full Text :
- https://doi.org/10.1002/bab.1506