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A highly selective ratiometric fluorescent and chromogenic probe for sulfite and its applications in imaging of living cells and zebrafish in vivo.
- Source :
-
Sensors & Actuators B: Chemical . Nov2017, Vol. 252, p86-94. 9p. - Publication Year :
- 2017
-
Abstract
- Herein we report a new carbazole-benzo[e]indolium dye (CEBI) as a ratiometric fluorescent and chromogenic probe for sulfite (SO 3 2− ) detection based on intramolecular charge transfer (ICT) mechanism. Upon treatment with SO 3 2− , the probe CEBI displayed a remarkable fluorescence ratiometric response (∼560-fold fluorescenc enhancement) together with a color change from red to weak red and a large shift (135 nm) in emission wavelength. The experimentally observed changes in the spectral features of the probe upon addition of SO 3 2− were systematically studied using high resolution electrospray ionization mass spectrometry (HR-ESI–MS), proton nuclear magnetic resonance ( 1 H NMR), and time-dependent density functional theory (TDDFT) calculations. We found a linear relationship between the ratio of fluorescence intensities at 465 nm and 600 nm ( F 465 /F 600 ) and SO 3 2− concentration over the range of 0–40 μM with a detection limit of 0.025 μM. Moreover, the novel probe demonstrated fluorescence imaging of SO 3 2− in living cells and zebrafish, suggesting its potential in clinical diagnostics. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 252
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 124820518
- Full Text :
- https://doi.org/10.1016/j.snb.2017.05.137