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Benzothiazole-Based Fluorescent Sensor for Ratiometric Detection of Zn(II) Ions and Secondary Sensing PPi and Its Applications for Biological Imaging and PPase Catalysis Assays.
- Source :
-
Industrial & Engineering Chemistry Research . Aug2017, Vol. 56 Issue 31, p8797-8805. 9p. - Publication Year :
- 2017
-
Abstract
- In this paper, we designed and synthesized three benzothiazole-based fluorescent probes L1, L2, and L3 for zinc ion detection. Among various metal ions, only the zinc ion exhibited fluorescence enhancement at 475 nm accompanied by the blue-shift emission wavelength in HEPES buffer solution containing probes. Through titration experiment, the detection limit of L1 for zinc ion sensing was calculated to be as low as 7 nM, which showed a high sensitivity. Furthermore, the confocal laser scanning micrographs of HeLa cells demonstrate good cell permeability of probe L1 and selective detection of zinc ion in living cells. The L1-Zn2+ complex was further used for pyrophosphate (PPi) sensing in HEPES buffer solution, the limit of detection was calculated to be as low as 60 nM. L1-Zn2+ can monitor the enzyme catalyzed degradation process of PPi, thus providing a meaningful way for tracking of zinc ion and pyrophosphate in biological systems. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BENZOTHIAZOLE
*IMAGING systems in biology
*ZINC ions
Subjects
Details
- Language :
- English
- ISSN :
- 08885885
- Volume :
- 56
- Issue :
- 31
- Database :
- Academic Search Index
- Journal :
- Industrial & Engineering Chemistry Research
- Publication Type :
- Academic Journal
- Accession number :
- 124886119
- Full Text :
- https://doi.org/10.1021/acs.iecr.7b01209