Back to Search Start Over

Puerarin promotes ABCA1-mediated cholesterol efflux and decreases cellular lipid accumulation in THP-1 macrophages.

Authors :
Li, Cong-hui
Gong, Duo
Chen, Ling-yan
Zhang, Min
Xia, Xiao-dan
Cheng, Hai-peng
Huang, Chong
Zhao, Zhen-wang
Zheng, Xi-Long
Tang, Xiao-er
Tang, Chao-ke
Source :
European Journal of Pharmacology. Sep2017, Vol. 811, p74-86. 13p.
Publication Year :
2017

Abstract

It was reported that puerarin decreases the total cholesterol, low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and increases high-density lipoprotein cholesterol (HDL-C) level, but the underlying mechanism is unclear. This study was designed to determine whether puerarin decreased lipid accumulation via up-regulation of ABCA1-mediated cholesterol efflux in THP-1 macrophage-derived foam cells. Our results showed that puerarin significantly promoted the expression of ATP-binding cassette transporter A1 (ABCA1) mRNA and protein via the AMP-activated protein kinase (AMPK)-peroxisome proliferator-activated receptor gamma (PPARγ)-liver X receptor-alpha (LXR-α) pathway and decreased cellular lipid accumulation in human THP-1 macrophage-derived foam cells. The miR-7 directly targeted 3′ untranslated region of STK11 (Serine/Threonine Kinase 11), which activated the AMPK pathway. Transfection with miR-7 mimic significantly reduced STK11 expression in puerarin-treated macrophages, decreased the phosphorylation of AMPK, down-regulated the expression of the PPARγ-LXR-α-ABCA1 expression. Additionally, treatment with miR-7 decreased cholesterol efflux and increased cholesterol levels in THP-1 macrophage-derived foam cells. Our study demonstrates that puerarin promotes ABCA1-mediated cholesterol efflux and decreases intracellular cholesterol levels through the pathway involving miR-7, STK11, and the AMPK-PPARγ-LXR-α-ABCA1 cascade. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00142999
Volume :
811
Database :
Academic Search Index
Journal :
European Journal of Pharmacology
Publication Type :
Academic Journal
Accession number :
124934286
Full Text :
https://doi.org/10.1016/j.ejphar.2017.05.055