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Development of a thermosensitive molecularly imprinted polymer resonance light scattering sensor for rapid and highly selective detection of hepatitis A virus in vitro.
- Source :
-
Sensors & Actuators B: Chemical . Dec2017, Vol. 253, p1188-1193. 6p. - Publication Year :
- 2017
-
Abstract
- In this study, a thermosensitive imprinted polymer coating SiO 2 was developed for a resonance light scattering (RLS) thermosensitive virus-affinity sensor, which exhibited high selectivity for virus monitoring. In this sensor, N -isopropylacrylamide was a temperature-sensitive element. The recognition performance of the nanosensor for viruses was regulated by temperature control for the specific capture of the target virus at 40 °C, resulting in the increase of RLS intensity and its release at 20 °C. Moreover, the RLS intensity of the nanosensor significantly increased within 30 min. Accordingly, a very good detection limit of 1.1 pmol L −1 was obtained. The nanosensor was successfully used to detect the additional hepatitis A virus from a dilution of human serum, and recoveries in the range of 90.8% to 108.3% were attained at three spiking levels of viruses. Overall, this study addressed the problems of high nonspecific adsorption and long duration of detection process. Furthermore, a facile, rapid, and efficient tool for virus detection was provided. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEPATITIS A
*VIRUSES
*DETECTORS
*LIGHT scattering
*RESONANCE
Subjects
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 253
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 125176307
- Full Text :
- https://doi.org/10.1016/j.snb.2017.07.166