AMPK 与脂肪细胞分化的关系研究及其在人脂肪源性肿瘤组织中 的表达特点.

Objective: In this study, we would emphatically study the role of AMPK in the process of adipocyte differentiation and dig out its possible relationship with Cidec during adipogenesis, in order to provide a potential role for AMPKα as a target in treating obesity or obesity-related diseases. Methods: Using IHC staining, real-time polymerase chain reaction (RT-PCR) and western blot analysis to detect AMPK and Cidec expression in different differentiation phase of human adipocytic tumors, as well as different developmental stages of human fetal adipose tissue. Results:The results showed that AMPK was gradually increased with a decrease in differentiation of the tumor cells, while Cidec was gradually decreased;and in the fetal adipose tissues, AMPK decreased (P＜0.01) and Cidec increased (P＜0.01) significantly with the birth age increased. It was implied that AMPK was closely related with adipocytes differentiation, and showed a reverse trend compared with Cidec during the differentiation course of human adipocytes. Conclusion:In this study, we demonstrated that AMPK played a key role during the differentiation of adipocytes, thus might propose important clues to discover new possible mechanism for human adipogenesis. [ABSTRACT FROM AUTHOR]