РОЛЬ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН ACINETOBACTER CALCOACETICUS ІМВ В-7241 У РУЙНУВАННІ БІОПЛІВОК.

Aim. To study the ability of surface-active agents (surfactants) synthesized Acinetobacter calcoaceticus IMV B-7241 on various substrates (ethanol, glycerol, n-hexadecane), destroy a biofilm certain bacteria and yeasts. Methods. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2: 1). The degree of biofilm destruction was determined as difference between the number of adhered cells in untreated and treated with surfactant holes of polystyrene immunological plate containing pre-formed biofilm of test cultures and was expressed as a percentage. The number of adherent cells was determined by spectrophotometrically. Results. The dependence of degree of biofilms destruction in the presence of A. calcoaceticus IMV B-7241 surfactants on the nature of carbon source in medium of producer cultivation, concentration of surfactants in the preparations and test culture type was established. Degree of Escherichia coli IEM-1 biofilm destruction in the presence of surfactants synthesized on all tested substrates practically did not differ and was 25-53% depending on the concentration of the surfactants (0,04-1,28 mg/ml). Bacillus subtilis BT-2 biofilm destruction reached 71-86% after addition of surfactants (0,16-0,64 mg/ml) obtained on ethanol and glycerol. Maximum destruction of Staphylococcus aureus BMS-1 biofilm (69-88%) and Candida albicans D-6 (42%) was observed in the presence of surfactants (0,32-1,28 mg/ml) synthesized on n-hexadecane. Conclusions. The effective concentrations of A. calcoaceticus IMV B-7241 surfactants, providing a high degree of bacterial biofilms destruction are comparable to those of the known microbial surfactants. The obtained data indicates the need for research the influence of producer cultivation conditions on the biological properties of final product. [ABSTRACT FROM AUTHOR]