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Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples.

Authors :
Liu, Liqiang
Chen, Yanni
Song, Shanshan
Zheng, Qiankun
Wu, Xiaoling
Kuang, Hua
Source :
Food & Agricultural Immunology. Nov2017, Vol. 28 Issue 6, p1283-1292. 10p.
Publication Year :
2017

Abstract

An immunochromatographic lateral flow strip test was developed for the detection of natamycin (Nata) residues in milk and cheese samples. Monoclonal antibody (mAb) against Nata was produced with a half maximal inhibitory concentration of 1.85 μg L−1. MAb conjugated with gold nanoparticles was the detection reagent. Nata conjugated with ovalbumin via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide method immobilized on a nitrocellulose membrane was the capture reagent. This semi-quantitative method required only 15 min to complete. The optimum concentrations of coating antigen and mAb were 0.15 µg mL−1and 0.2 µg mL−1, respectively. Based on an optical density scanner, the visual limit of detection of Nata was 5 μg L−1and 10 μg kg−1in milk and yoghurt samples, respectively. [ABSTRACT FROM PUBLISHER]

Details

Language :
English
ISSN :
09540105
Volume :
28
Issue :
6
Database :
Academic Search Index
Journal :
Food & Agricultural Immunology
Publication Type :
Academic Journal
Accession number :
125434484
Full Text :
https://doi.org/10.1080/09540105.2017.1337084