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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP).

Authors :
Sun, Yuanyuan
Tian, Hui
Liu, Chenghui
Sun, Yueying
Li, Zhengping
Source :
Chemical Communications. 10/14/2017, Vol. 53 Issue 80, p11040-11043. 4p.
Publication Year :
2017

Abstract

A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13597345
Volume :
53
Issue :
80
Database :
Academic Search Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
125506634
Full Text :
https://doi.org/10.1039/c7cc06140d