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Zoledronic acid sensitizes rhabdomyosarcoma cells to cytolysis mediated by human γδ T cells.
- Source :
-
Oncology Letters . Nov2017, Vol. 14 Issue 5, p5597-5604. 8p. - Publication Year :
- 2017
-
Abstract
- Rhabdomyosarcoma (RMS) is the most common type of soft-tissue sarcoma in children. Immunotherapy has been proposed as a treatment for this deadly tumor. In the present study, the cytotoxicity of ex vivo expanded γδ T cells on RMS cell lines was evaluated and the molecular interactions involved were investigated. γδ T cells were expanded in vitro using peripheral blood mononuclear cells from 5 healthy donors and were stimulated with zoledronic acid (Zol) and interleukin 2. RMS cell lines RD and A-673 were used as target cells. The cytotoxicity of the γδ T cells against RMS was assessed in vitro and in vivo. γδ T cells were cytotoxic to RMS cells. Importantly, Zol markedly increased their cytotoxic potential. RMS cells treated with Zol-stimulated γδ T cells to produce interferon γ. γδ T cell-mediated cytotoxicity was primarily through the T cell receptor-dependent signaling pathway in blocking studies. Transfer of γδ T cells together with Zol into nude mice induced the regression of RD tumor xenotransplants. The results of the present study provide the rationale for the clinical evaluation of γδ T cells in RMS. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ZOLEDRONIC acid
*RHABDOMYOSARCOMA
*CANCER cells
*T cells
*CANCER immunotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 17921074
- Volume :
- 14
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Oncology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 125520106
- Full Text :
- https://doi.org/10.3892/ol.2017.6894