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Mechanism of myocardial ischemia/reperfusion-induced acute kidney injury through DJ-1/Nrf2 pathway in diabetic rats.
- Source :
-
Experimental & Therapeutic Medicine . Nov2017, Vol. 14 Issue 5, p4201-4207. 7p. - Publication Year :
- 2017
-
Abstract
- The objective of the present study was to investigate acute kidney injury (AKI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats and elucidate its underlying mechanism. A rat model of MIR was established by left anterior descending coronary artery occlusion for 30 min, followed by reperfusion for 2 h. Rats were randomly divided into four groups: i) Sham group, ii) sham + MIR group, iii) diabetic group and iv) diabetes + MIR group. Myocardial injury was detected by plasma creatine kinase isoenzyme MB and lactate dehydrogenase assays. AKI induced by MIR in diabetic rats was characterized by increases in cystatin C and ƒÀ2.microglobulin levels. Oxidative stress injury was accompanied by an increase of malondialdehyde levels and a decrease of total antioxidative capacity in the renal tissues. Immunohistochemistry and western blot analysis demonstrated that the expression of DJ.1 and nuclear factor erythroid 2.related factor 2 (Nrf2) were significantly increased in the diabetes + MIR group compared with that in the sham + MIR and diabetic groups. Taken together, these results suggested that AKI induced by MIR in diabetic rats may be associated with activation of the DJ.1/Nrf2 pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CORONARY disease
*CORONARY artery bypass
*CREATINE kinase
*DIABETES
*ISOENZYMES
Subjects
Details
- Language :
- English
- ISSN :
- 17920981
- Volume :
- 14
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Experimental & Therapeutic Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 125534412
- Full Text :
- https://doi.org/10.3892/etm.2017.5095