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Generation of an infectious clone of AMDV and identification of capsid residues essential for infectivity in cell culture.
- Source :
-
Virus Research . Oct2017, Vol. 242, p58-65. 8p. - Publication Year :
- 2017
-
Abstract
- Pathogenic strains of Aleutian mink disease virus (AMDV) such as Utah-1 do not replicate in cell culture (e.g., Crandell Rees feline kidney cells) while the in vitro- adapted AMDV strain ADV-Gorham (ADV-G) is not pathogenic. Here, we constructed a full-length infectious clone (pADV-G). Alignment of the VP2 gene of ADV-G with that of other AMDV strains revealed many amino acid (a.a.) residues conserved among pathogenic isolates that differed in ADV-G. Four virulence-associated, conserved residues of pADV-G VP2 were studied by site-directed mutagenesis (H92A, Q94S, Y115F, and I116L). Mutation of residue 92 or 94 decreased viral-transcription and viral-infectivity levels, whereas mutation of residue 115 or 116 did not affect viral-infectivity in CRFK cells. These results indicated that VP2 residues 92 and 94, both located on the surface of the viral capsid, are critical for AMDV infectivity in vitro . [ABSTRACT FROM AUTHOR]
- Subjects :
- *MINK Aleutian disease
*CAPSIDS
*CELL culture
*VIRUS virulence
*MUTAGENESIS
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 242
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 125781881
- Full Text :
- https://doi.org/10.1016/j.virusres.2017.09.011